Phase I of the FDM Bioprinter incorporated a 70% ethanol wash for sterilization of the scaffold. This process however only creates an aseptic environment for the cells and does not prevent bacterial growth. Therefore, Phase II of the Bioprinter will incorporate a novel design for UV sterilization in the device which both destroys harmful pathogens and prevents bacterial growth.
Testing:
The scaffold will be tested using a Trypticase soy medium which supports microbial growth. If microbial growth occurs then the method of sterilization is not sufficient.
Testing:
The scaffold will be tested using a Trypticase soy medium which supports microbial growth. If microbial growth occurs then the method of sterilization is not sufficient.